HA211048

Product name:	Human IL-3R alpha/CD123, C-His Tag
Catalog No.:	HA211048
Bio-Activity:	Testing in progress.
Purity:	>95% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific α subunit (IL-3Rα) and a signal transducing β subunit (IL-3Rβ) common to the IL-3/IL-5/GM-CSF receptors. The α subunits are low-affinity ligand-binding proteins while the β subunits do not themselves bind ligand, but are required for high affinity binding by the α subunits. The mouse IL-3 receptor has two distinct β subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine β subunits are 91% homologous at the amino acid level but only 56% homologous to the human β subunit. The carboxy-terminus of the β subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.