HA211081

Product name:	Human I-309/CCL1, Tag Free
Catalog No.:	HA211081
Bio-Activity:	Testing in progress.
Purity:	>90% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Chemokine (C-C motif) ligand 1 (CCL1) is also known as small inducible cytokine A1 and I-309 in humans. CCL1 is a small glycoprotein that belongs to the CC chemokine family. CCL1 is a small glycoprotein with a molecular weight of approximately 15-16 kDa. CCL1 is secreted by activated monocytes/macrophages, T lymphocytes and endothelial cells. CCL1 binds to the chemokine receptor CCR8 and induces Ca2+ influx, chemotaxis and regulate apoptosis. CCR8 is constitutively expressed in monocytes/macrophages, Th2, and regulatory T lymphocytes. Thus, CCL1 mainly acts as a chemoattractant for monocytes/macrophages, T lymphocytes, specially Th2-differentiated T cells and a subset of T regulatory cells in vitro into inflammatory site. It can also attract NK cells, immature B cells but does not attract neutrophils. CCL1 stimulates a transient increase in the concentration of cytoplasmic free calcium in monocytes but not in other type of cells. Furthermore, CCL1 inhibits apoptosis in thymic cell lines by the RAS/MAPK pathway but can prevent dexamethasone-induced apoptosis in cultured murine thymic lymphoma cells.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.