Mouse EGF, Tag Free Protein (Active)
cat.: HA211220
Product name: Mouse EGF, Tag Free
Catalog No.: HA211220
Bio-Activity: Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.06-0.24 ng/mL.
Purity: >95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Background: Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein originally found in rodents and humans. It has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGFα and vaccinia virus growth factor exhibit 30-40% amino acid homology. Several additional members of the EGF/TGF family have been described; these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor. Amphiregulin and the heparin-binding EGF-like growth factor both bind to the EGF receptor.
Source: HEK293
Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution: Reconstitute at 250 μg/ml in sterile water.
Images
HA211220_1.jpg Fig1: Protein on SDS-PAGE under reducing (R) condition.
HA211220_2.jpg Fig2: Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.06-0.24 ng/mL.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.