Human Beta-NGF, Tag Free Protein
cat.: HA211342
Product name: Human Beta-NGF, Tag Free
Catalog No.: HA211342
Bio-Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.4-1.6 ng/mL.
Purity: >95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Background: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.
Source: HEK293
Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution: Reconstitute at 250 μg/ml in sterile water.
Images
HA211342_1.jpg Fig1: Protein on SDS-PAGE under reducing (R) condition.
HA211342_2.jpg Fig2: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.4-1.6 ng/mL.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.