HA211391

Product name:	Human Granzyme K, Tag Free
Catalog No.:	HA211391
Bio-Activity:	Testing in progress.
Purity:	>95% as determined by SDS-PAGE.
Endotoxin:	Less than 1.0 EU per μg by the LAL method.
Formulation:	Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Background:

Granzyme K (GzmK) is a serine protease that sits in the dense-core granules of cytotoxic T lymphocytes and NK cells. While its gene neighbors encode granzymes A–C, GzmK stands out by lacking the N-linked glycosylation motifs that decorate most family members; this minimalist design keeps it stable at the low pH of the granule and allows it to emerge in an immediately active form once perforin has punched entry holes in a target cell. Inside the cytosol, GzmK bypasses the canonical caspase cascade: it clips Bid to tBid, nicks the anti-apoptotic SET complex, and generates single-stranded DNA nicks that push the cell into a swift form of death intermediate between apoptosis and necrosis. In addition, any GzmK that leaks into the extracellular space can process pro-IL-1β and activate PAR-2 on macrophages and endothelial cells, turning a private hit into a broader inflammatory alert.

Source:

HEK293

Storage:

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution:

Reconstitute at 250 μg/ml in sterile water.