Human phosphorylated Tau231 Protein
cat.: HA211531
Product name: Human phosphorylated Tau231
Catalog No.: HA211531
Purity: >95% as determined by SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Background: Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Tau is highly subject to a variety of post-translational modifications, including phosphorylation on serine and threonine residues, polyubiquitination (and subsequent proteasomal degradation) and glycation of specific Tau isoforms. Defects in the gene encoding Tau are associated with Alzheimers disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP).
Source: HEK293
Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution: Reconstitute at 250 μg/ml in sterile water.
Images
HA211531_1.jpg Fig1: Protein on SDS-PAGE under reducing (R) condition.
HA211531_2.jpg Fig2: Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of Human phosphorylated Tau231 diluted in carbonate/bicarbonate buffer, at a concentration of 1µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 100 µl 1% BSA/TBS blocking buffer at 37℃ for 1 hours, and incubated with serial diluted detect antibodies starting from 4,000 pg/ml to 3.907 pg/ml for 45 minutes at 37℃ with shaking. Then the plate was washed and incubated with 50 µl per well of second antibody for 30 minutes at 37℃ with shaking. Detection was performed for 10 minutes at 37℃ in the dark. The reaction was stopped and absorbances were read on a spectrophotometer at 450 nm.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.