PSMA7 Rabbit Polyclonal Antibody
cat.: HA500004
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMA7 aa 50-248. |
| Positive control: | SKBR-3 cell lysate, HepG2 cell lysate, Hela cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, human liver carcinoma tissue, human pancreas tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleus, Proteasome. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: O14818 Human | Q9Z2U0 Mouse | P48004 Rat |
| Alternative names: | C6 HSPC MGC3755 OTTHUMP00000031449 OTTHUMP00000031450 OTTHUMP00000031453 Proteasome (prosome macropain) subunit alpha type 7 Proteasome alpha 7 subunit Proteasome subunit alpha 4 Proteasome subunit alpha type 7 Proteasome subunit alpha type-7 Proteasome subunit RC6 1 Proteasome subunit RC6-1 Proteasome subunit XAPC7 PSA7_HUMAN PSMA 7 PSMA7 RC6 1 XAPC7 |
Images
|
Fig1:
Western blot analysis of PSMA7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500004, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SKBR-3 cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse lung tissue lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PSMA7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500004, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSMA7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500004, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of Hela cells labeling PSMA7. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500004, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.