Dopamine Transporter Rabbit Polyclonal Antibody
cat.: HA500007
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 68 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human Dopamine Transporter aa 570-620.
Positive control: Mouse brian tissue lysates, rat brain tissue, mouse brain tissue, SKOV-3, F9.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:1,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q01959 Human | Q61327 Mouse | P23977 Rat
Alternative names: DA transporter DAT 1 DAT DAT1 Dopamine transporter 1 Dopamine transporter PKDYS SC6A3_HUMAN SLC6A3 Sodium dependent dopamine transporter Sodium-dependent dopamine transporter Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 Solute carrier family 6 (neurotransmitter transporter), member 3 Solute carrier family 6 member 3 Variable number tandem repeat (VNTR)
Images
HA500007_1.jpg Fig1: Western blot analysis of Dopamine Transporter on mouse brian tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500007, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500007_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Dopamine Transporter antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500007, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500007_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Dopamine Transporter antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500007, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500007_4.jpg Fig4: ICC staining of Dopamine Transporter in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500007, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500007_5.jpg Fig5: Flow cytometric analysis of Dopamine Transporter was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500007, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.