Rad51B Rabbit Polyclonal Antibody
cat.: HA500010
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human Rad51B. |
| Positive control: | SKBR3 cell lysate, PC-12 cell lysate, SiHa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: O15315 Human |
| Alternative names: | DNA repair protein RAD51 homolog 2 hREC2 MGC34245 OTTHUMP00000212251 OTTHUMP00000212253 OTTHUMP00000212254 OTTHUMP00000212255 R51H2 RA51B_HUMAN RAD51 homolog B (S. cerevisiae) RAD51 homolog B RAD51 like 1 RAD51 like protein 1 RAD51-like protein 1 Rad51B RAD51L1 REC2 RecA like protein Recombination repair protein |
Images
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Fig1:
Western blot analysis of Rad51B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SKBR3 cell lysate Lane 2: PC-12 cell lysate |
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Fig2:
Flow cytometric analysis of SiHa cells labeling Rad51B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500010, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.