HPD Rabbit Polyclonal Antibody
cat.: HA500012
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45/40 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HPD aa 1-200. |
| Positive control: | Rat liver tissue lysate, human kidney tissue lysate, human liver tissue, mouse liver tissue, rat kidney tissue. |
| Subcellular location: | Cytosol, endoplasmic reticulum membrane, extracellular exosome, golgi membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P32754 Human | P49429 Mouse | P32755 Rat |
| Alternative names: | 4 HPPD 4 hydroxyphenylpyruvate dioxygenase 4 hydroxyphenylpyruvic acid oxidase 4-hydroxyphenylpyruvate dioxygenase 4-hydroxyphenylpyruvic acid oxidase 4HPPD GLOD3 glyoxalase domain containing 3 HPD HPPD_HUMAN HPPDase PPD |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HPD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500012, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HPD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500012, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HPD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500012, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Western blot analysis of HPD on different lysates with Rabbit anti-HPD antibody (HA500012) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 120 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500012, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.