TAG1 Rabbit Polyclonal Antibody
cat.: HA500013
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 113 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TAG1 aa 40-230. |
| Positive control: | Mouse cerebellum tissue lysate, human brain tissue lysate, rat cerebellum tissue lysate, F9. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q02246 Human | Q61330 Mouse | P22063 Rat |
| Alternative names: | Axonal glycoprotein TAG 1 Axonal glycoprotein TAG-1 Axonal glycoprotein TAG1 Axonin 1 Axonin-1 Axonin1 AXT CNTN 2 Cntn2 CNTN2_HUMAN contactin 2 (axonal) Contactin 2 Contactin-2 Contactin2 DKFZp781D102 MGC157722 TAG 1 TAG 564 TAG564 TAX 1 TAX TAX-1 TAX1 Transient axonal glycoprotein 1 Transiently expressed axonal glycoprotein |
Images
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Fig1:
Western blot analysis of TAG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500013, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse cerebellum tissue lysate Lane 2: Human brain tissue lysate Lane 3: Rat cerebellum tissue lysate |
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Fig2: ICC staining of TAG1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500013, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.