SPTBN1 Rabbit Polyclonal Antibody
cat.: HA500014
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 275 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SPTBN1 aa 2320-2364. |
| Positive control: | SW1990 cell lysate, PANC-1 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, rat kidney tissue, human liver carcinoma tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:800 |
| Uniprot #: | SwissProt: Q01082 Human | Q62261 Mouse |
| Alternative names: | Beta G spectrin Beta II spectrin Beta spectrin 2 Beta spectrin II Beta-II spectrin betaSpII brain 1 ELF Embryonic liver beta fodrin Fodrin beta chain non-erythroid beta chain 1 Spectrin Spectrin beta chain Spectrin, beta, non erythrocytic 1 Spectrin, non erythroid beta chain 1 SPTB2 SPTB2_HUMAN SPTBN1 |
Images
|
Fig1:
Western blot analysis of SPTBN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500014, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW1990 cell lysate Lane 2: PANC-1 cell lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse cerebellum tissue lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SPTBN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500014, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SPTBN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500014, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SPTBN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500014, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.