SPTBN1 Rabbit Polyclonal Antibody
cat.: HA500014
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 275 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SPTBN1 aa 2320-2364. |
| Positive control: | SW1990 cell lysate, PANC-1 cell lysate, Rat brain tissue lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, SW1990. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoskeleton, Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q01082 Human | Q62261 Mouse Entrez Gene: 305614 Rat |
| Alternative names: | Beta G spectrin Beta II spectrin Beta spectrin 2 Beta spectrin II Beta-II spectrin betaSpII brain 1 ELF Embryonic liver beta fodrin Fodrin beta chain non-erythroid beta chain 1 Spectrin Spectrin beta chain Spectrin, beta, non erythrocytic 1 Spectrin, non erythroid beta chain 1 SPTB2 SPTB2_HUMAN SPTBN1 |
Images
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Fig1:
Western blot analysis of SPTBN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500014, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW1990 cell lysate Lane 2: PANC-1 cell lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse cerebellum tissue lysate |
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Fig2:
Immunocytochemistry analysis of SW1990 cells labeling SPTBN1 with Rabbit anti-SPTBN1 antibody (HA500014) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SPTBN1 antibody (HA500014) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SW1990 cells labeling SPTBN1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500014, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.