BRCA1 Rabbit Polyclonal Antibody
cat.: HA500015
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa-208 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BRCA1 aa 1-200. |
| Positive control: | Daudi cell lysate, mouse testis tissue. |
| Subcellular location: | Nucleus, Chromosome, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P38398 Human | P48754 Mouse | O54952 Rat |
| Alternative names: | BRCA 1 BRCA1 BRCA1 DNA repair associated BRCA1/BRCA2 containing complex subunit 1 BRCA1/BRCA2-containing complex, subunit 1 BRCA1_HUMAN BRCAI BRCC 1 BRCC1 Breast and ovarian cancer susceptibility protein 1 Breast Cancer 1 Breast Cancer 1 Early Onset Breast cancer type 1 susceptibility protein BROVCA1 FANCS IRIS PNCA4 PPP1R53 Protein phosphatase 1 regulatory subunit 53 PSCP RING finger protein 53 RNF53 |
Images
|
Fig1: Western blot analysis of BRCA1 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500015, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRCA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500015, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.