TAT Rabbit Polyclonal Antibody
cat.: HA500016
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TAT aa 1-200. |
| Positive control: | HL-60 cell lysate, Daudi cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Cytosol, mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P17735 Human | Q8QZR1 Mouse | P04694 Rat |
| Alternative names: | ATTY_HUMAN L-tyrosine:2-oxoglutarate aminotransferase TAT Tyrosine aminotransferase Tyrosine aminotransferase, cytosolic |
Images
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Fig1:
Western blot analysis of TAT on different lysates with Rabbit anti-TAT antibody (HA500016) at 1/2,000 dilution. Lane 1: Raji (Human burkitt lymphoma cell) cell lysate Lane 2: HepG2 (Human liver cancer cell) cell lysate Lane 3: SH-SY5Y (Human derived cell) cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse heart tissue lysate Lane 7: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 14 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500016, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.