GKAP Rabbit Polyclonal Antibody
cat.: HA500018
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 70 kDa-109 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human GKAP aa 380-430.
Positive control: Human brain tissue cell lysates, PC-12, N2A, SHG-44, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue, Daudi.
Subcellular location: Cell junction, Cell membrane, Membrane, Synapse.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O14490 Human | Q9D415 Mouse | P97836 Rat
Alternative names: 4933422O14Rik 9630002F18 AI845682 AI848168 BB075781 D17Bwg0511e DAP 1 ALPHA DAP 1 DAP 1 BETA DAP-1 DAP-1-ALPHA DAP-1-BETA DAP1 Discs large (Drosophila) homolog associated protein 1 Discs large homolog associated protein 1 Disks large associated protein 1 Disks large associated protein 1, alpha isoform, included Disks large associated protein 1, beta isoform, included Disks large-associated protein 1 DLGAP 1 Dlgap1 DLGP1_HUMAN GKAP GKAP/SAPAP GKPA/SAPAP Guanylate kinase associated protein Guanylate kinase-associated protein hGKAP KIAA4162 MGC88156 mKIAA4162 PSD 95/SAP90 binding protein 1 PSD-95/SAP90-binding protein 1 SAP90/PSD 95 associated protein 1 SAP90/PSD-95-associated protein 1 SAPAP 1 SAPAP1
Images
HA500018_1.jpg Fig1: Western blot analysis of GKAP on human brain tissue cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500018_2.jpg Fig2: ICC staining of GKAP in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500018, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500018_3.jpg Fig3: ICC staining of GKAP in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500018, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500018_4.jpg Fig4: ICC staining of GKAP in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500018, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500018_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-GKAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500018_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GKAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500018_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GKAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500018, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500018_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GKAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500018, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500018_9.jpg Fig9: Flow cytometric analysis of GKAP was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500018, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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