PDCD6 Rabbit Polyclonal Antibody
cat.: HA500019
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PDCD6 aa 1-191. |
| Positive control: | SK-Br-3 cell lysate, HepG2 cell lysate, rat ovary tissue lysate, human breast carcinoma tissue, human placenta tissue, human stomach carcinoma tissue. |
| Subcellular location: | Endosome, Endoplasmic reticulum membrane, Nucleus, COPII-coated vesicle membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: O75340 Human | G3V7W1 Rat |
| Alternative names: | AIP1 ALG 2 ALG-2-interacting protein 1 ALG2 ALIX Apoptosis linked gene 2 Apoptosis linked gene 2 protein Apoptosis-linked gene 2 protein FLJ42309 FLJ46208 Hp95 KIAA1375 MA 3 MA3 MGC111017 MGC119050 MGC9123 PDCD 6 Pdcd6 PDCD6_HUMAN PEF 1B PEF1B Probable calcium binding protein ALG 2 Probable calcium binding protein ALG2 Probable calcium-binding protein ALG-2 Programmed cell death 6 Programmed cell death 6-interacting protein Programmed cell death protein 6 PS 2 PS2 |
Images
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Fig1:
Western blot analysis of PDCD6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500019, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: HepG2 cell lysate Lane 1: Rat ovary tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDCD6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500019, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PDCD6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500019, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PDCD6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500019, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.