Beta-galactosidase Rabbit Polyclonal Antibody
cat.: HA500021
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Beta-galactosidase aa 610-677. |
| Positive control: | A549 cell lysate, Hela cell lysate, human lung carcinoma tissue, LOVO, A549, mouse kidney tissue, rat epididymis tissue. |
| Subcellular location: | Cytoplasm, Lysosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50 1:400-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P16278 Human | P23780 Mouse Entrez Gene: 316033 Rat |
| Alternative names: | Acid beta galactosidase Acid beta-galactosidase Beta galactosidase 1 Beta galactosidase Beta-galactosidase BGAL_HUMAN EBP EBP, included Elastin receptor 1 (67kD) Elastin receptor 1 67kDa Elastin receptor 1 Elastin receptor 1, included Elastin-binding protein, included ELNR1 Galactosidase beta 1 GLB 1 GLB1 Lactase MPS4B S-GAL, included |
Images
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Fig1:
Western blot analysis of Beta-galactosidase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500021, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Hela cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Beta-galactosidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500021, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of LOVO cells labeling Beta-galactosidase with Rabbit anti-Beta-galactosidase antibody (HA500021) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Beta-galactosidase antibody (HA500021) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of A549 cells labeling Beta-galactosidase. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500021, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Beta-galactosidase antibody (HA500021) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Beta-galactosidase antibody (HA500021) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.