MEMO1 Rabbit Polyclonal Antibody
cat.: HA500024
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MEMO1 aa 1-200. |
| Positive control: | Mouse lung tissue lysate, mouse colon tissue lysate, mouse liver tissue, human skeletal muscle tissue, rat large intestine tissue. |
| Subcellular location: | Cytosol, nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q9Y316 Human | Q91VH6 Mouse | Q4QQR9 Rat |
| Alternative names: | C21orf19-like protein C2orf4 CGI 27 DKFZp434I0135 FLJ25031 HCV NS5A-transactivated protein 7 Hepatitis C virus NS5A-transactivated protein 7 Mediator of cell motility 1 Mediator of ErbB2-driven cell motility 1 MEMO Memo-1 memo1 MEMO1_HUMAN NS5ATP7 Protein MEMO1 |
Images
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Fig1:
Western blot analysis of MEMO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500024, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: Mouse colon tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MEMO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.