MEMO1 Rabbit Polyclonal Antibody
cat.: HA500024
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MEMO1 aa 1-200. |
| Positive control: | Mouse lung tissue lysate, Mouse colon tissue lysate, human colon tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Cytosol, nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:5,000 |
| Uniprot #: | SwissProt: Q9Y316 Human | Q91VH6 Mouse | Q4QQR9 Rat |
| Alternative names: | C21orf19-like protein C2orf4 CGI 27 DKFZp434I0135 FLJ25031 HCV NS5A-transactivated protein 7 Hepatitis C virus NS5A-transactivated protein 7 Mediator of cell motility 1 Mediator of ErbB2-driven cell motility 1 MEMO Memo-1 memo1 MEMO1_HUMAN NS5ATP7 Protein MEMO1 |
Images
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Fig1:
Western blot analysis of MEMO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500024, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: Mouse colon tissue lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MEMO1 antibody (HA500024) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-MEMO1 antibody (HA500024) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-MEMO1 antibody (HA500024) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500024) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.