UGGT1 Rabbit Polyclonal Antibody
cat.: HA500025
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 177 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human UGGT1 aa 1350-1555. |
| Positive control: | HL-60 cell lysate, HepG2 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat brain tissue lysate, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9NYU2 Human | Q6P5E4 Mouse | Q9JLA3 Rat |
| Alternative names: | GT HUGT1 UDP glucose glycoprotein glucosyltransferase 1 UDP Glucose Glycoprotein Glucosyltransferase UDP--Glc:glycoprotein glucosyltransferase UDP-glucose ceramide glucosyltransferase-like 1 UDP-glucose:glycoprotein glucosyltransferase 1 UGCGL1 UGGG1 UGGG1_HUMAN UGGT Uggt1 UGT1 UGTR |
Images
|
Fig1:
Western blot analysis of UGGT1 on different lysates with Rabbit anti-UGGT1 antibody (HA500025) at 1/1,000 dilution. Lane 1: HL-60 cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 177 kDa Observed band size: 177 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500025) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UGGT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.