UGGT1 Rabbit Polyclonal Antibody
cat.: HA500025
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 177 kDa
Isotype: IgG
Immunogen: Recombinant protein within human UGGT1 aa 1350-1555.
Positive control: HL-60 cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9NYU2 Human | Q6P5E4 Mouse | Q9JLA3 Rat
Alternative names: GT HUGT1 UDP glucose glycoprotein glucosyltransferase 1 UDP Glucose Glycoprotein Glucosyltransferase UDP--Glc:glycoprotein glucosyltransferase UDP-glucose ceramide glucosyltransferase-like 1 UDP-glucose:glycoprotein glucosyltransferase 1 UGCGL1 UGGG1 UGGG1_HUMAN UGGT Uggt1 UGT1 UGTR
Images
HA500025_1.jpg Fig1: Western blot analysis of UGGT1 on different lysates with Rabbit anti-UGGT1 antibody (HA500025) at 1/1,000 dilution.

Lane 1: HL-60 cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: Mouse liver tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (20 µg/Lane)
Lane 6: Rat brain tissue lysate (20 µg/Lane)

Predicted band size: 177 kDa
Observed band size: 177 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500025) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.