ENAH Rabbit Polyclonal Antibody
cat.: HA500026
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human ENAH. |
| Positive control: | SiHa cell lysate, Hela cell lysate, 293T cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, MG-63, SiHa. |
| Subcellular location: | Cytoskeleton, cytoplasm, lamellipodium, filopodium, synapse, focal adhesion. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8N8S7 Human | Q03173 Mouse |
| Alternative names: | ENA Enah ENAH_HUMAN MENA NDPP1 Protein enabled homolog |
Images
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Fig1:
Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500026, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate |
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Fig2:
Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500026, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Rat brain tissue lysate |
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Fig3:
Immunocytochemistry analysis of MG-63 cells labeling ENAH with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Flow cytometric analysis of SiHa cells labeling ENAH. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500026, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.