ENAH Rabbit Polyclonal Antibody
cat.: HA500026
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human ENAH. |
| Positive control: | SiHa cell lysate, Hela cell lysate, 293T cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, MG-63, SiHa. |
| Subcellular location: | Cytoskeleton, cytoplasm, lamellipodium, filopodium, synapse, focal adhesion. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8N8S7 Human | Q03173 Mouse |
| Alternative names: | ENA Enah ENAH_HUMAN MENA NDPP1 Protein enabled homolog |
Images
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Fig1:
Western blot analysis of ENAH on different lysates with Rabbit anti-ENAH antibody (HA500026) at 1/1,000 dilution. Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate Lane 2: C6 (Rat glioma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500026, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 66.5 kDa Observed band size: 67kDa |
|
Fig2:
Immunocytochemistry analysis of MG-63 cells labeling ENAH with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Flow cytometric analysis of SiHa cells labeling ENAH. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500026, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.