MAT2A Rabbit Polyclonal Antibody
cat.: HA500028
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 44 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MAT2A aa 70-260.
Positive control: Rat kidney tissue lysates, rat kidney tissue, human colon tissue, human colon carcinoma tissue, mouse brain tissue, Hela.
Subcellular location: Nucleoplasm, Cytosol.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:500-1:2,000
1:500-1:1,000
Uniprot #: SwissProt: P31153 Human | Q3THS6 Mouse | P18298 Rat
Alternative names: AdoMet synthase 2 AdoMet synthetase 2 AdoMet synthetase AMS 2 AMS2 MAT 2 MAT 2A MAT II MAT-II Mat2a MATA 2 MATA2 MATII Methionine adenosyltransferase 2 Methionine adenosyltransferase Methionine adenosyltransferase II alpha Methionine adenosyltransferase II METK2_HUMAN S adenosylmethionine synthetase gamma form S adenosylmethionine synthetase isoform type 2 S-adenosylmethionine synthase isoform type-2 SAMS 2 SAMS2
Images
HA500028_1.jpg Fig1: Western blot analysis of MAT2A on rat kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500028, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500028_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-MAT2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500028, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500028_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MAT2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500028, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500028_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MAT2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500028, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500028_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAT2A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500028, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500028_6.jpg Fig6: Flow cytometric analysis of Hela cells labeling MAT2A.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500028, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.