p50 dynamitin Rabbit Polyclonal Antibody
cat.: HA500029
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human p50 dynamitin aa 1-200. |
| Positive control: | SHSY5Y cell lysate, Mouse brain tissue lysate, SH-SY5Y, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:200-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q13561 Human | Q99KJ8 Mouse | Q6AYH5 Rat |
| Alternative names: | 50 kD dynein associated polypeptide 50 kDa dynein associated polypeptide 50 kDa dynein-associated polypeptide DCTN-50 DCTN2 DCTN2_HUMAN DCTN50 dynactin 2 (p50) dynactin 2 dynactin complex 50 kD subunit Dynactin complex 50 kDa subunit Dynactin subunit 2 DYNAMITIN p50 dynamitin RBP50 |
Images
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Fig1:
Western blot analysis of p50 dynamitin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500029, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY5Y cell lysate Lane 2: Mouse brain tissue lysate |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling p50 dynamitin with Rabbit anti-p50 dynamitin antibody (HA500029) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p50 dynamitin antibody (HA500029) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-p50 dynamitin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500029, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of SH-SY5Y cells labeling p50 dynamitin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500029, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.