| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Pig |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CRMP4 1-50. |
| Positive control: | SH-SY5Y cell lysate, Pig brain cell lysate, Mouse brain cell lysate, Rat brain cell lysate. |
| Subcellular location: | Cell projection, Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000-1:10,000 |
| Uniprot #: | SwissProt: Q14195 Human | Q62188 Mouse | Q62952 Rat |
| Alternative names: | Collapsin response mediator protein 4 Collapsin response mediator protein 4 long CRMP 4 CRMP-4 CRMP4 Dihydropyrimidinase like 3 Dihydropyrimidinase related protein 3 Dihydropyrimidinase-related protein 3 DPYL3_HUMAN DPYSL3 DRP 3 DRP-3 DRP3 LCRMP TUC4 ULIP 1 ULIP ULIP-1 ULIP1 UNc 33 like phosphoprotein 1 Unc 33 like phosphoprotein Unc-33-like phosphoprotein 1 |
|
Fig1:
Western blot analysis of CRMP4 on different lysates with Rabbit anti-CRMP4 antibody (HA500030) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate, 15 µg/Lane Lane 2: pig brain cell lysate, 30 µg/Lane Lane 3: mouse brain cell lysate, 30 µg/Lane Lane 3: rat brain cell lysate, 30 µg/Lane Exposure time: 8 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500030, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 62 kDa Observed band size: 62 kDa |