MST1 Rabbit Polyclonal Antibody
cat.: HA500031
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 57 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human MST1 aa 400-450.
Positive control: Mouse bone marrow tissue lysates, human tonsil tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:500-1:2,000
Uniprot #: SwissProt: Q13043 Human | Q9JI11 Mouse
Alternative names: Kinase responsive to stress Krs2 Mammalian STE20 like protein kinase 1 Mammalian STE20-like protein kinase 1 Mammalian sterile 20 like 1 MST-1 MST1 Serine/threonine kinase 4 Serine/threonine protein kinase Krs 2 Serine/threonine-protein kinase 4 Serine/threonine-protein kinase Krs-2 STE20 like kinase MST1 STE20-like kinase MST1 STK4 STK4_HUMAN TIIAC YSK3
Images
HA500031_1.jpg Fig1: Western blot analysis of MST1 on mouse bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500031, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500031_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MST1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500031, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.