Dynamin 3 Rabbit Polyclonal Antibody
cat.: HA500032
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 97 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Dynamin 3 aa 820-869. |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, Mouse brain tissue lysate,Rat brain tissue lysate, mouse brain tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UQ16 Human | Q8BZ98 Mouse | Q08877 Rat |
| Alternative names: | testicular dynamin testicular DNM 3 DNM3 DNM3 protein Dyn3 DYN3_HUMAN Dyna III DynaIII dynamin 2 Dynamin Dynamin family member Dynamin testicular Dynamin-3 Dynamin3 EC 3.6.5.5 KIAA0820 MGC70433 T dynamin |
Images
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Fig1:
Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA500032) at 1/5,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500032, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97 kDa Observed band size: 97 kDa |
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Fig2:
Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA500032) at 1/5,000 dilution. Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500032, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97 kDa Observed band size: 97 kDa |
|
Fig3:
Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA500032) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brian tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500032, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97 kDa Observed band size: 97 kDa |
|
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Dynamin 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500032, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.