Dynamin 3 Rabbit Polyclonal Antibody
cat.: HA500037
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Dynamin 3 aa 750-800. |
| Positive control: | Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, SKOV-3. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UQ16 Human | Q08877 Rat |
| Alternative names: | testicular dynamin testicular DNM 3 DNM3 DNM3 protein Dyn3 DYN3_HUMAN Dyna III DynaIII dynamin 2 Dynamin Dynamin family member Dynamin testicular Dynamin-3 Dynamin3 EC 3.6.5.5 KIAA0820 MGC70433 T dynamin T-dynamin |
Images
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Fig1:
Western blot analysis of Dynamin 3 on different lysates with Rabbit anti-Dynamin 3 antibody (HA500037) at 1/5,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse small intestine tissue lysate Lane 3: Rat cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 59 seconds; ECL: K1801 The expression level on Mouse small intestine (low) is consistent with literature (PMID: 21689597). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500037, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97.7 kDa Observed band size: 98 kDa |
|
Fig2: ICC staining of Dynamin 3 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500037, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.