Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 79 kDa. |
Isotype: | IgG |
Immunogen: | Recombinant protein within human GFPT1 aa 1-200. |
Positive control: | 293 cell lysate, rat testis tissue lysate, mouse liver tissue lysate, LOVO, HepG2, rat kidney tissue, human liver tissue, mouse skeletal muscle tissue. |
Subcellular location: | cytosol, extracellular exosome. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: Q06210 Human | P47856 Mouse | P82808 Rat |
Alternative names: | CMS12 CMSTA1 D-fructose-6-phosphate amidotransferase 1 GFA GFAT 1 GFAT GFAT1 GFAT1m GFPT Gfpt1 GFPT1_HUMAN GFPT1L Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1 Glutamine--fructose-6-phosphate transaminase 1 Glutamine:fructose 6 phosphate amidotransferase 1 Hexosephosphate aminotransferase 1 MSLG |
Fig1:
Western blot analysis of GFPT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500038, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: Rat testis tissue lysate Lane 3: Mouse liver tissue lysate |
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Fig2: ICC staining of GFPT1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500038, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig3: ICC staining of GFPT1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500038, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). | |
Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GFPT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500038, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GFPT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500038, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-GFPT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500038, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig7:
Western blot analysis of GFPT1 on different lysates with Rabbit anti-GFPT1 antibody (HA500038) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si GFPT1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500038) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |