OAS3 Rabbit Polyclonal Antibody
cat.: HA500039
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 121 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OAS3 aa 900-1059. |
| Positive control: | A549 cell lysate, human placental tissue lysate, A431 cell lysate, human skin tissue, LOVO cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y6K5 Human |
| Alternative names: | (2-5'')oligo(A) synthase 3 2 5' oligo(A) synthetase 3 2 5'LIGO 2 5A synthetase 3 2''-5''-oligoadenylate synthase 3 2-5A synthase 3 MGC133260 OAS3 2' 5' oligoadenylate synthetase 3, 100kDa OAS3 OAS3_HUMAN p100 p100 OAS p100OAS |
Images
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Fig1:
Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500039, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Human placental tissue lysate Lane 3: A431 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-OAS3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500039, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of A594 cells labeling OAS3 with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of LOVO cells labeling OAS3 with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.