OAS3 Rabbit Polyclonal Antibody
cat.: HA500039
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 121 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OAS3 aa 900-1059. |
| Positive control: | Mouse testis tissue lysate, Rat testis tissue lysate, A549 cell lysate, Human placental tissue lysate, A431 cell lysate, human testis tissue, A594, LOVO. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y6K5 Human | Q8VI93 Mouse | Q5MYT7 Rat |
| Alternative names: | (2-5'')oligo(A) synthase 3 2 5' oligo(A) synthetase 3 2 5'LIGO 2 5A synthetase 3 2''-5''-oligoadenylate synthase 3 2-5A synthase 3 MGC133260 OAS3 2' 5' oligoadenylate synthetase 3, 100kDa OAS3 OAS3_HUMAN p100 p100 OAS p100OAS |
Images
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Fig1:
Western blot analysis of OAS3 on different lysates with Rabbit anti-OAS3 antibody (HA500039) at 1/1,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 126 kDa Observed band size: 126 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500039, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: Human placental tissue lysate Lane 3: A431 cell lysate |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-OAS3 antibody (HA500039) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500039) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of A594 cells labeling OAS3 with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of LOVO cells labeling OAS3 with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-OAS3 antibody (HA500039) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.