STK4 / MST-1 Rabbit Polyclonal Antibody
cat.: HA500043
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human STK4 aa 1-50. |
| Positive control: | HepG2 cell lysate, THP-1 cell lysate, rat kidney tissue, human spleen tissue, human placenta tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q13043 Human | Q9JI11 Mouse |
| Alternative names: | Kinase responsive to stress Krs2 Mammalian STE20 like protein kinase 1 Mammalian STE20-like protein kinase 1 Mammalian sterile 20 like 1 MST-1 MST1 Serine/threonine kinase 4 Serine/threonine protein kinase Krs 2 Serine/threonine-protein kinase 4 Serine/threonine-protein kinase Krs-2 STE20 like kinase MST1 STE20-like kinase MST1 STK4 STK4_HUMAN TIIAC YSK3 |
Images
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Fig1:
Western blot analysis of STK4 / MST-1 on different lysates with Rabbit anti-STK4 / MST-1 antibody (HA500043) at 1/5,00 dilution. Lane 1: HepG2 Lane 2: Jurkat Lane 3: A431 Lane 4: Rat kidney Lysates/proteins at 15 µg/Lane. Exposure time: 8 secondss; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500043, 1/5,00 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55.6 kDa Observed band size: 56 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-STK4 / MST-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500043, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STK4 / MST-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500043, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STK4 / MST-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500043, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STK4 / MST-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500043, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.