S100A2 Rabbit Polyclonal Antibody
cat.: HA500044
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 11 kDa
Isotype: IgG
Immunogen: Recombinant protein within human S100A2 aa 1-98.
Positive control: A431 cell lysates, rat bladder tissue, human skin tissue, human esophagus tissue, mouse kidney tissue.
Subcellular location: Nucleoplasm, Cytosol , Nucleoli, Plasma membrane.
Recommended Dilutions:
  WB
  IHC-P
1:5,000-1:10,000
1:200-1:1,000
Uniprot #: SwissProt: P29034 Human
Alternative names: CAN19 MGC111539 Protein S 100L Protein S-100L Protein S100 A2 Protein S100-A2 Protein S100L S100 A2 S100 calcium binding protein A2 S100 calcium-binding protein A2 S100A2 S100L S10A2_HUMAN
Images
HA500044_1.jpg Fig1: Western blot analysis of S100A2 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500044, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500044_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500044_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500044_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500044_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.