S100A2 Rabbit Polyclonal Antibody
cat.: HA500044
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human S100A2 aa 1-98. |
| Positive control: | A431 cell lysates, rat bladder tissue, human skin tissue, human esophagus tissue, mouse kidney tissue. |
| Subcellular location: | Nucleoplasm, Cytosol , Nucleoli, Plasma membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000-1:10,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P29034 Human |
| Alternative names: | CAN19 MGC111539 Protein S 100L Protein S-100L Protein S100 A2 Protein S100-A2 Protein S100L S100 A2 S100 calcium binding protein A2 S100 calcium-binding protein A2 S100A2 S100L S10A2_HUMAN |
Images
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Fig1:
Western blot analysis of S100A2 on A431 (Human epidermoid carcinoma skin squamous cell) cell lysate with Rabbit anti-S100A2 antibody (HA500044) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 8 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500044, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 11 kDa Observed band size: 11 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100A2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500044, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.