Gemin 2 Rabbit Polyclonal Antibody
cat.: HA500045
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Gemin 2 aa 230-280. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, Mouse testis tissue lysate, Mouse thymus tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue. |
| Subcellular location: | Nucleus, gem, Cytoplasm |
| Recommended Dilutions:
WB IHC-P |
1:10,000 1:1,000 |
| Uniprot #: | SwissProt: O14893 Human | Q9CQQ4 Mouse |
| Alternative names: | Component of gems 2 Gem (nuclear organelle) associated protein 2 Gem associated protein 2 GEMI2_HUMAN Gemin-2 gemin2 SIP 1 SIP-1 SIP1 SIP1 delta SIP1-delta SMN interacting protein 1 SMN interacting protein 1 delta SMN-interacting protein 1 Survival interacting protein 1 Survival of motor neuron protein interacting protein 1 Survival of motor neuron protein-interacting protein 1 |
Images
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Fig1:
Western blot analysis of Gemin 2 on different lysates with Rabbit anti-Gemin 2 antibody (HA500045) at 1/10,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500045) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Gemin 2 antibody (HA500045) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500045) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Gemin 2 antibody (HA500045) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500045) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.