Histone H3 (acetyl K18) Rabbit Polyclonal Antibody
cat.: HA500047
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Histone H3 (acetyl K18) aa 1-50. |
| Positive control: | Hela cell lysate , untreated, Hela cell lysate, treated with Sodium Butyrate 0.5mM for 24h, Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h, mouse colon, HeLa cells treated with 500ng/mL Trichostatin A for 4 hours. |
| Subcellular location: | Chromosome, Nucleosome core, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:100-1:500 1:500 |
| Uniprot #: | SwissProt: P68431 Human | P84243 Human | Q16695 Human | Q71DI3 Human | Q6NXT2 Human |
| Alternative names: | H3 histone family member E pseudogene H3 histone family, member A H3/A H31_HUMAN H3F3 H3FA Hist1h3a HIST1H3B HIST1H3C HIST1H3D HIST1H3E HIST1H3F HIST1H3G HIST1H3H HIST1H3I HIST1H3J HIST3H3 histone 1, H3a Histone cluster 1, H3a Histone H3 3 pseudogene Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l H3K18ac |
Images
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Fig1:
Western blot analysis of Histone H3 (acetyl K18) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500047, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with Sodium Butyrate 0.5mM for 24h |
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Fig2:
Western blot analysis of Histone H3 (acetyl K18) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500047, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H3 (acetyl K18) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500047, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells treated with 500ng/mL Trichostatin A for 4 hours labeling Histone H3 (acetyl K18) with Rabbit anti-Histone H3 (acetyl K18) antibody (HA500047) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (acetyl K18) antibody (HA500047) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.