OTC/Ornithine Carbamoyltransferase Rabbit Polyclonal Antibody
cat.: HA500048
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OTC/Ornithine Carbamoyltransferase aa 200-354. |
| Positive control: | Rat colon tissue lysate, human liver tissue lysate, mouse liver tissue lysate, SW480, human liver carcinoma tissue, human liver tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:50-1:200 1:100-1:500 |
| Uniprot #: | SwissProt: P00480 Human | P11725 Mouse | P00481 Rat |
| Alternative names: | EC 2.1.3.3 MGC129967 MGC129968 MGC138856 OCTD Ornithine Carbamoyltransferase Ornithine carbamoyltransferase mitochondrial Ornithine carbamoyltransferase, mitochondrial Ornithine transcarbamylase Otc OTC_HUMAN OTCase |
Images
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Fig1: ICC staining of OTC/Ornithine Carbamoyltransferase in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500048, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-OTC/Ornithine Carbamoyltransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500048, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OTC/Ornithine Carbamoyltransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500048, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of OTC/Ornithine Carbamoyltransferase on human liver tissue lysate with Rabbit anti-OTC/Ornithine Carbamoyltransferase antibody (HA500048) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500048, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39.9 kDa Observed band size: 40 kDa |
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Fig5:
Western blot analysis of OTC/Ornithine Carbamoyltransferase on Mouse liver tissue lysate with Rabbit anti-OTC/Ornithine Carbamoyltransferase antibody (HA500048) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500048, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39.9 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.