mCherry / TdTomato Rabbit Polyclonal Antibody
cat.: HA500049
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mCherry 1-236aa. |
| Positive control: | Recombinant mCherry protein lysates. |
| Recommended Dilutions:
WB ELISA IF-Cell |
1:5,000-1:80,000 1:1,000-1:5,000 1:10,000 |
| Uniprot #: | SwissProt: X5DSL3 AnaplasmaMarginale |
Images
|
Fig1:
Western blot analysis of mCherry / TdTomato on different lysates with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/5,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mCherry / TdTomato cell lysate Lysates/proteins at 5 µg/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500049) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of mCherry / TdTomato on tdTomato recombinant protein with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/80,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500049) at 1/80,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: ELISA analysis of with anti-mCherry antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-mCherry antibody was used as the capture antigen for ELISA. |
|
Fig4:
Immunocytochemistry analysis of HeLa cells transfected with or without mCherry labeling mCherry / TdTomato with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-mCherry / TdTomato antibody (HA500049) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.