ADK Rabbit Polyclonal Antibody
cat.: HA500050
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ADK aa 1-200. |
| Positive control: | A431 cell lysate, mouse testis tissue lysate, rat kidney tissue lysate, human placenta tissue, human colon carcinoma tissue, human lung carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P55263 Human | P55264 Mouse | Q64640 Rat |
| Alternative names: | 2310026J05Rik 5033405D03Rik Adenosine 5''-phosphotransferase Adenosine kinase adk ADK_HUMAN AI255373 AI987814 AK MGC6593 OTTHUMP00000019864 OTTHUMP00000019865 |
Images
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Fig1:
Western blot analysis of ADK on different lysates with Rabbit anti-ADK antibody (HA500050) at 1/2,000 dilution. Lane 1: A431 cell lysate (10ug/lane) Lane 2: mouse testis tissue lysate (15ug/lane) Lane 3: rat kidney tissue lysate (15ug/lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500050, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG-HRP(HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 41 kDa Observed band size: 41 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ADK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500050, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ADK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500050, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-ADK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500050, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.