ASNA1 Rabbit Polyclonal Antibody
cat.: HA500051
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ASNA1 aa 300-348. |
| Positive control: | 293T cell lysate, Rat testis tissue lysate, Rat brain tissue lysate, 293T. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: O43681 Human | G3V9T7 Rat |
| Alternative names: | ARSA 1 ARSA ArsA arsenite transporter ATP binding homolog 1 ArsA arsenite transporter, ATP binding, E. coli, homolog of, 1 ArsA arsenite transporter, ATP-binding, homolog 1 (bacterial) ARSA I ARSA-I ARSA1 ARSAI Arsenical pump driving ATPase Arsenical pump-driving ATPase Arsenical resistance ATPase Arsenite translocating ATPase Arsenite transporting ATPase Arsenite-stimulated ATPase ASNA 1 ASNA I ASNA-I ASNA_HUMAN ASNA1 Asna1 protein ASNAI ATPase ASNA1 GET3 Golgi to ER traffic 3 homolog hARSA-I hASNA-I MGC3821 Transmembrane domain recognition complex 40 kDa ATPase subunit Transmembrane domain recognition complex, 40kDa TRC40 |
Images
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Fig1:
Western blot analysis of ASNA1 on different lysates with Rabbit anti-ASNA1 antibody (HA500051) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: Rat testis tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 35 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500051, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 38.8kDa Observed band size: 39 kDa |
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Fig2:
Immunocytochemistry analysis of 293T cells labeling ASNA1 with Rabbit anti-ASNA1 antibody (HA500051) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ASNA1 antibody (HA500051) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of 293T cells labeling ASNA1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500051, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.