ASNA1 Rabbit Polyclonal Antibody
cat.: HA500051
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ASNA1 aa 300-348. |
| Positive control: | MCF-7 cell lysate, 293T cell lysate, rat kidney tissue, human thyroid tissue, human pancreas tissue, human rectal cancer tissue. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: O43681 Human | G3V9T7 Rat |
| Alternative names: | ARSA 1 ARSA ArsA arsenite transporter ATP binding homolog 1 ArsA arsenite transporter, ATP binding, E. coli, homolog of, 1 ArsA arsenite transporter, ATP-binding, homolog 1 (bacterial) ARSA I ARSA-I ARSA1 ARSAI Arsenical pump driving ATPase Arsenical pump-driving ATPase Arsenical resistance ATPase Arsenite translocating ATPase Arsenite transporting ATPase Arsenite-stimulated ATPase ASNA 1 ASNA I ASNA-I ASNA_HUMAN ASNA1 Asna1 protein ASNAI ATPase ASNA1 GET3 Golgi to ER traffic 3 homolog hARSA-I hASNA-I MGC3821 Transmembrane domain recognition complex 40 kDa ATPase subunit Transmembrane domain recognition complex, 40kDa TRC40 |
Images
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Fig1:
Western blot analysis of ASNA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500051, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: 293T cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ASNA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500051, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ASNA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500051, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ASNA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500051, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue using anti-ASNA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500051, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.