Smg1 Rabbit Polyclonal Antibody
cat.: HA500054
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 410/395/340/269 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Smg1 aa 3450-3661. |
| Positive control: | Hela cell lysates, human kidney tissue, human skeletal muscle tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q96Q15 Human |
| Alternative names: | 61E3.4 ATX hSMG-1 hSMG1 KIAA0421 Lambda interacting protein Lambda-interacting protein Lambda/iota protein kinase C interacting protein Lambda/iota protein kinase C-interacting protein LIP Phosphatidylinositol 3 kinase related kinase Phosphatidylinositol 3 kinase related protein kinase PI 3 kinase related kinase SMG 1 Serine/threonine protein kinase SMG1 Serine/threonine-protein kinase SMG1 SMG-1 smg1 SMG1_HUMAN |
Images
|
Fig1: Western blot analysis of Smg1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500054, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Smg1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500054, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Smg1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500054, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.