PDE2A Rabbit Polyclonal Antibody
cat.: HA500055
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PDE2A aa 750-941. |
| Positive control: | Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, human kidney tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000-1:50,000 1:1,000 |
| Uniprot #: | SwissProt: O00408 Human | Q922S4 Mouse |
| Alternative names: | 5''-cyclic phosphodiesterase cGMP dependent 3'5' cyclic phosphodiesterase cGMP stimulated phosphodiesterase 1 cGMP stimulated phosphodiesterase 4 cGMP-dependent 3'' cGMP-stimulated phosphodiesterase 2 CGS PDE CGS-PDE cGSPDE Cyclic GMP stimulated phosphodiesterase Cyclic GMP-stimulated phosphodiesterase Human cGMP stimulated 3'5' cyclic nucleotide phosphodiesterase PDE 2A PDE 2A1 PDE 2A4 PDE2A PDE2A_HUMAN PDE2A1 PDE2A4 phosphodiesterase 2A Phosphodiesterase 2A cGMP stimulated |
Images
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Fig1:
Western blot analysis of PDE2A on different lysates with Rabbit anti-PDE2A antibody (HA500055) at 1/50,000 dilution. Lane 1: mouse spleen tissue lysate Lane 2: mouse brain tissue lysate Lane 3: rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500055, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 105.7 kDa Observed band size: 105 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PDE2A antibody (HA500055) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500055) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PDE2A antibody (HA500055) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500055) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.