SMURF1 Rabbit Polyclonal Antibody
cat.: HA500057
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size:86 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SMURF1 aa 30-80. |
| Positive control: | NIH/3T3 cell lysate, MCF-7 cell lysate, rat kidney tissue lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9HCE7 Human | Q9CUN6 Mouse |
| Alternative names: | E3 ubiquitin-protein ligase SMURF1 hSMURF1 KIAA1625 Smad specific E3 ubiquitin ligase 1 SMAD specific E3 ubiquitin protein ligase 1 Smad ubiquitination regulatory factor 1 SMAD-specific E3 ubiquitin-protein ligase 1 SMUF1_HUMAN SMURF 1 smurf1 |
Images
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Fig1:
Western blot analysis of SMURF1 on different lysates with Rabbit anti-SMURF1 antibody (HA500057) at 1/1,000 dilution. Lane 1: MCF-7 (Human breast cancer cell) cell lysate Lane 2: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 62 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500057, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted MW: 86 kDa Observed MW: 86 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.