GDF1 Rabbit Polyclonal Antibody
cat.: HA500058
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human GDF1. |
| Positive control: | SH-SY5Y cell lysate, MCF-7 cell lysate, rat brain tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:100-1:500 |
| Uniprot #: | SwissProt: P27539 Human |
| Alternative names: | DORV DTGA3 Embryonic growth/differentiation factor 1 GDF 1 GDF-1 GDF1 GDF1_HUMAN Growth differentiation factor 1 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GDF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500058, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Western blot analysis of GDF1 on different lysates with Rabbit anti-GDF1 antibody (HA500058) at 1/5,000 dilution. Lane 1: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 100 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500058, 1/5,000 in 5% NFDM/TBST Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39.5 kDa Observed band size: 50 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.