Cadherin 10 Rabbit Polyclonal Antibody
cat.: HA500059
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human Cadherin 10. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, Mouse hippocampus tissue lysate, Rat hippocampus tissue lysate, SH-SY5Y cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9Y6N8 Human | P70408 Mouse Entrez Gene: 29181 Rat |
| Alternative names: | CAD10_HUMAN Cadherin 10 (T2 cadherin) Cadherin 10 type 2 (T2 cadherin) Cadherin 10 type 2 Cadherin-10 Cadherin10 CDH10 T2 cadherin T2-cadherin |
Images
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Fig1:
Western blot analysis of Cadherin 10 on different lysates with Rabbit anti-Cadherin 10 antibody (HA500059) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Rat cerebellum tissue lysate Lane 5: Mouse hippocampus tissue lysate Lane 6: Rat hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500059, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 88 kDa Observed band size: 130 kDa |
|
Fig2: Western blot analysis of Cadherin 10 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500059, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.