TMPRSS2 Rabbit Polyclonal Antibody
cat.: HA500060
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TMPRSS2 aa 300-350. |
| Positive control: | PC-3M cell lysate, mouse colon tissue lysate, human stomach tissue, human pancreas tissue. |
| Subcellular location: | Cell membrane, Membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,1000 |
| Uniprot #: | SwissProt: O15393 Human | Q9JIQ8 Mouse |
| Alternative names: | D16Ertd61e Epitheliasin FLJ41954 MGC6821 PP9284 PRSS10 Serine protease 10 TMPRSS2 TMPRSS2 ERG FUSION GENE, INCLUDED TMPRSS2 ETV1 FUSION GENE, INCLUDED TMPS2_HUMAN Transmembrane protease serine 2 catalytic chain Transmembrane protease, serine 2 Transmembrane protease, serine 2, EC 3.4.219 |
Images
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Fig1:
Western blot analysis of TMPRSS2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500060, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3M cell lysate Lane 2: Mouse colon tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-TMPRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500060, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TMPRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500060, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.