| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Strep-tag II. |
| Positive control: | Strep-Tag II protein transfected 293F cells lysate, Non-transfected 293F cells lysate. |
| Recommended Dilutions:
WB |
1:5,000-1:40,000 |
| Alternative names: | anti-Strep-Tag II Strep-Tag II Strep-Tag II Strep-Tag IIantiboy |
|
Fig1:
Western blot analysis of Strep-Tag II on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500061, 1/40,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Strep-Tag II protein transfected 293F cells lysate Lane 2: Non-transfected 293F cells lysate |