Strep-Tag II Rabbit Polyclonal Antibody
cat.: HA500061
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Strep-tag II. |
| Positive control: | 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate. |
| Recommended Dilutions:
WB |
1:1,000-1:40,000 |
| Alternative names: | anti-Strep-Tag II Strep-Tag II Strep-Tag II Strep-Tag IIantiboy |
Images
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Fig1:
Western blot analysis of Strep-Tag II on 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate with Rabbit anti-Strep-Tag II antibody (HA500061) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500061, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.