Scramblase 1 Rabbit Polyclonal Antibody
cat.: HA500063
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Scramblase 1. |
| Positive control: | SK-MEL-28 cell lysate, HeLa cell lysate, THP-1 cell lysate, MDA-MB-231 cell lysate, HUVEC, human tonsil tissue, SK-MEL-28. |
| Subcellular location: | Cell membrane, Nucleus, Membrane, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IP |
1:1,000 1:50-1:100 1:50-1:200 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: O15162 Human |
| Alternative names: | Ca(2+) dependent phospholipid scramblase 1 Ca(2+)-dependent phospholipid scramblase 1 Erythrocyte phospholipid scramblase MM1 cell-derived transplantability-associated gene 1b, mouse, homolog of MmTRA1a MmTRA1b Nor1 Phospholipid scramblase 1 PL scramblase 1 PLS1_HUMAN PLSCR 1 Plscr1 Scramblase1 Tra1 Tra1a Tra1b Transplantability-associated protein 1 Tras1 Tras2 |
Images
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Fig1:
Western blot analysis of Scramblase 1 on different lysates with Rabbit anti-Scramblase 1 antibody (HA500063) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HeLa cell lysate Lane 3: THP-1 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: SH-SY5Y cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500063) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Scramblase 1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500063, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Scramblase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500063, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of SK-MEL-28 cells labeling Scramblase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500063, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Scramblase 1 was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA500063 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA500063 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-MEL-28 cell lysate (input) Lane 2: HA500063 IP in SK-MEL-28 cell lysate Lane 3: Rabbit IgG instead of HA500063 in SK-MEL-28 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 14 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.