Scramblase 1 Rabbit Polyclonal Antibody
cat.: HA500063
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Scramblase 1. |
| Positive control: | SK-MEL-28 cell lysate, HeLa cell lysate, THP-1 cell lysate, MDA-MB-231 cell lysate, HUVEC, SK-MEL-28. |
| Subcellular location: | Cell membrane, Nucleus, Membrane, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:1,000 1:50-1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: O15162 Human |
| Alternative names: | Ca(2+) dependent phospholipid scramblase 1 Ca(2+)-dependent phospholipid scramblase 1 Erythrocyte phospholipid scramblase MM1 cell-derived transplantability-associated gene 1b, mouse, homolog of MmTRA1a MmTRA1b Nor1 Phospholipid scramblase 1 PL scramblase 1 PLS1_HUMAN PLSCR 1 Plscr1 Scramblase1 Tra1 Tra1a Tra1b Transplantability-associated protein 1 Tras1 Tras2 |
Images
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Fig1:
Western blot analysis of Scramblase 1 on different lysates with Rabbit anti-Scramblase 1 antibody (HA500063) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HeLa cell lysate Lane 3: THP-1 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: SH-SY5Y cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500063) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Scramblase 1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500063, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Flow cytometric analysis of SK-MEL-28 cells labeling Scramblase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500063, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Scramblase 1 was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA500063 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA500063 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-MEL-28 cell lysate (input) Lane 2: HA500063 IP in SK-MEL-28 cell lysate Lane 3: Rabbit IgG instead of HA500063 in SK-MEL-28 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 14 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.