FOXG1 Rabbit Polyclonal Antibody
cat.: HA500064
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size:52 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FOXG1 aa 430-485. |
| Positive control: | Mouse brain tissue lysate, SHSY5Y cell lysate, SHG-44 cell lysate, human skin tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P55316 Human | Q60987 Mouse |
| Alternative names: | BF-1 BF-2 BF1 BF2 Brain factor 1 Brain factor 2 FHKL FKH2 FKHL1 FKHL2 FKHL3 FKHL4 Forkhead box protein G1 Forkhead box protein G1A Forkhead box protein G1B Forkhead box protein G1C Forkhead like 1 Forkhead like 2 Forkhead like 3 Forkhead like 4 Forkhead-related protein FKHL1 Forkhead-related protein FKHL2 Forkhead-related protein FKHL3 FOXG1 FOXG1_HUMAN FOXG1A FOXG1B FOXG1C HBF 1 HBF G2 hBF-2 HBF2 HFK1 HFK2 HFK3 KHL2 Oncogene QIN QIN |
Images
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Fig1:
Western blot analysis of FOXG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500064, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: SHSY5Y cell lysate Lane 2: SHG-44 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-FOXG1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500064, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.