Estrogen Sulfotransferase Rabbit Polyclonal Antibody
cat.: HA500065
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Estrogen Sulfotransferase aa 150-294. |
| Positive control: | Human kidney tissue lysates, rat kidney tissue lysates, human small intestine tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P49888 Human | P52844 Rat |
| Alternative names: | EST EST-1 EST1 Estrogen sulfotransferase estrogen-preferring estrone sulfotransferase ST1E1 ST1E1_HUMAN STE Sulfotransferase 1E1 Sulfotransferase Sulfotransferase estrogen preferring Sulfotransferase family 1E member 1 SULT1E1 |
Images
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Fig1:
Western blot analysis of Estrogen Sulfotransferase on Mouse kidney tissue lysates with Rabbit anti-Estrogen Sulfotransferase antibody (HA500065) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500065, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 35.1 kDa Observed band size: 35.1 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Estrogen Sulfotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500065, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.