ARF4 Rabbit Polyclonal Antibody
cat.: HA500066
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human ARF4. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, mouse kidney tissue lysate, mouse testis tissue lysate, mouse ovary tissue lysate, rat testis tissue lysate, F9, human breast carcinoma tissue, mouse kidney tissue. |
| Subcellular location: | Golgi apparatus. Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:50-1:100 1:200-1:1,000 |
| Uniprot #: | SwissProt: P18085 Human | P61750 Mouse | P61751 Rat |
| Alternative names: | ADP ribosylation factor 2 ADP ribosylation factor 4 ADP-ribosylation factor 4 ARF2 ARF4 ARF4_HUMAN |
Images
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Fig1:
Western blot analysis of ARF4 on different lysates with Rabbit anti-ARF4 antibody (HA500066) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (30 µg/Lane) Lane 4: Mouse testis tissue lysate (30 µg/Lane) Lane 5: Mouse ovary tissue lysate (30 µg/Lane) Lane 6: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 21 kDa Observed band size: 16 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500066) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of ARF4 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500066, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ARF4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500066, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ARF4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500066, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.