RhoGDI Rabbit Polyclonal Antibody
cat.: HA500067
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RhoGDI aa 50-204.
Positive control: HepG2 cell lysate, SH-SY5Y cell lysate, rat lung tissue lysate, MG-63, SH-SY5Y, rat testis tissue, human lung tissue, human thyroid tissue, mouse thyroid tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P52565 Human | Q99PT1 Mouse | Q5XI73 Rat
Alternative names: ARHGDIA fa96g11 GDIA 1 GDIA1 GDIR1_HUMAN MGC117248 NPHS8 Rho GDI 1 Rho GDI alpha Rho GDI Rho GDP dissociation inhibitor (GDI) alpha Rho GDP dissociation inhibitor 1 Rho GDP dissociation inhibitor alpha Rho GDP-dissociation inhibitor 1 Rho-GDI alpha RhoGDI 1 RhoGDI alpha RHOGDI RhoGDI1 wu:fa96g11 zgc:55554 zgc:77681
Images
HA500067_1.jpg Fig1: Western blot analysis of RhoGDI on different lysates with Rabbit anti-RhoGDI antibody (HA500067) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD RhoGDI cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 5 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500067) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500067_2.jpg Fig2: Western blot analysis of RhoGDI on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500067, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Rat lung tissue lysate
HA500067_3.jpg Fig3: ICC staining of RhoGDI in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500067, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500067_4.jpg Fig4: ICC staining of RhoGDI in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500067, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
HA500067_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500067_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500067_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500067_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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