RhoGDI Rabbit Polyclonal Antibody
cat.: HA500067
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RhoGDI aa 50-204.
Positive control: A549 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, human lung tissue, human thyroid tissue, mouse thyroid tissue, rat testis tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
1:5,000
1:50-1:200
Uniprot #: SwissProt: P52565 Human | Q99PT1 Mouse | Q5XI73 Rat
Alternative names: ARHGDIA fa96g11 GDIA 1 GDIA1 GDIR1_HUMAN MGC117248 NPHS8 Rho GDI 1 Rho GDI alpha Rho GDI Rho GDP dissociation inhibitor (GDI) alpha Rho GDP dissociation inhibitor 1 Rho GDP dissociation inhibitor alpha Rho GDP-dissociation inhibitor 1 Rho-GDI alpha RhoGDI 1 RhoGDI alpha RHOGDI RhoGDI1 wu:fa96g11 zgc:55554 zgc:77681
Images
HA500067_1.jpg Fig1: Western blot analysis of RhoGDI on different lysates with Rabbit anti-RhoGDI antibody (HA500067) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD RhoGDI cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 5 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500067) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500067_2.jpg Fig2: Western blot analysis of RhoGDI on different lysates with Rabbit anti-RhoGDI antibody (HA500067) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: Neuro-2a cell lysate
Lane 4: C6 cell lysate
Lane 5: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 8 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500067) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA500067_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500067_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500067_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500067_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RhoGDI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500067, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.