PHLDA1 Rabbit Polyclonal Antibody
cat.: HA500068
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TDAG51 aa 20-60. |
| Positive control: | Mouse brain tissue lysates, human lung carcinoma tissue, human breast tissue, human esophagus tissue, mouse brain tissue. |
| Subcellular location: | Nucleolus. Cytoplasm. Cytoplasmic vesicle |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: Q8WV24 Human | Q62392 Mouse |
| Alternative names: | Apoptosis associated nuclear protein Apoptosis-associated nuclear protein DT1P1B11 MGC131738 PHLA1_HUMAN PHLDA1 PHRIP Pleckstrin homology like domain family A member 1 Pleckstrin homology-like domain family A member 1 PQ rich protein PQ-rich protein PQR protein Proline- and glutamine-rich protein Proline- and histidine-rich protein T cell death associated gene T CELL DEATH ASSOCIATED GENE 51 T-cell death-associated gene 51 protein Tdag TDAG51 |
Images
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Fig1:
Western blot analysis of PHLDA1 on mouse brain tissue lysate with Rabbit anti-PHLDA1 antibody (HA500068) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500068, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 45 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.