PHLDA1 Rabbit Polyclonal Antibody
cat.: HA500068
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human TDAG51 aa 20-60.
Positive control: Mouse brain tissue lysates, human lung carcinoma tissue, human breast tissue, human esophagus tissue, mouse brain tissue.
Subcellular location: Nucleolus. Cytoplasm. Cytoplasmic vesicle
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:100-1:500
Uniprot #: SwissProt: Q8WV24 Human | Q62392 Mouse
Alternative names: Apoptosis associated nuclear protein Apoptosis-associated nuclear protein DT1P1B11 MGC131738 PHLA1_HUMAN PHLDA1 PHRIP Pleckstrin homology like domain family A member 1 Pleckstrin homology-like domain family A member 1 PQ rich protein PQ-rich protein PQR protein Proline- and glutamine-rich protein Proline- and histidine-rich protein T cell death associated gene T CELL DEATH ASSOCIATED GENE 51 T-cell death-associated gene 51 protein Tdag TDAG51
Images
HA500068_1.jpg Fig1: Western blot analysis of PHLDA1 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500068, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
HA500068_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500068_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500068_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA500068_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHLDA1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500068, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.