CHMP2A Rabbit Polyclonal Antibody
cat.: HA500070
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size:25 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human CHMP2A. |
| Positive control: | SW480 cell lysate, A431 cell lysate, HepG2 cell lysate, rat bladder tissue. |
| Subcellular location: | Late endosome membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:100 1:200-1:1,000 |
| Uniprot #: | SwissProt: O43633 Human | Q9DB34 Mouse | B2RZB5 Rat |
| Alternative names: | BC 2 BC2 Charged multivesicular body protein 2a CHM2A_HUMAN CHMP2 CHMP2a Chromatin modifying protein 2a Chromatin-modifying protein 2a hVps2 1 hVps2-1 putative breast adenocarcinoma marker Putative breast adenocarcinoma marker BC-2 Putative breast adenocarcinoma marker BC2 Vacuolar protein sorting associated protein 2 1 Vacuolar protein sorting-associated protein 2-1 Vps2 1 VPS2 Vps2-1 VPS2A |
Images
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Fig1:
Western blot analysis of CHMP2A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500070, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: HepG2 cell lysate Lane 4: SW480 cell lysate |
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Fig2: ICC staining of CHMP2A in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500070, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-CHMP2A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500070, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.